A freshwater cyanophage whose genome indicates close relationships to photosynthetic marine cyanomyophages Public Deposited

http://ir.library.oregonstate.edu/concern/defaults/fn107034w

This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Society for Applied Microbiology and Blackwell Publishing Ltd and published by John Wiley & Sons Ltd. It can be found at:  http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291462-2920.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Bacteriophage S-CRM01 has been isolated from a freshwater strain of Synechococcus and shown to be present in the upper Klamath River valley in northern California and Oregon. The genome of this lytic T4-like phage has a 178,563 bp circular genetic map with 297 predicted protein-coding genes and 33 tRNA genes that represent all 20 amino acid specificities. Analyses based on gene sequence and gene content indicate a close phylogenetic relationship to the “photosynthetic” marine cyanomyophages infecting Synechococcus and Prochlorococcus. Such relatedness suggests that freshwater and marine phages can draw on a common gene pool. The genome can be considered as being comprised of three regions. Region 1 is populated predominantly with structural genes, recognized as such by homology to other T4-like phages and by identification in a proteomic analysis of purified virions. Region 2 contains most of the genes with roles in replication, recombination, nucleotide metabolism and regulation of gene expression, as well as 5 of the 6 signature genes of the photosynthetic cyanomyophages (hli03, hsp20, mazG, phoH and psbA; cobS is present in Region 3). Much of Regions 1 and 2 are syntenous with marine cyanomyophage genomes, except that a segment encompassing Region 2 is inverted. Region 3 contains a high proportion (85%) of genes that are unique to S-CRM01, as well as most of the tRNA genes. Regions 1 and 2 contain many predicted late promoters, with a combination of CTAAATA and ATAAATA core sequences. Two predicted genes that are unusual in phage genomes are homologs of cellular spoT and nusG.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Dreher, T. W., Brown, N., Bozarth, C. S., Schwartz, A. D., Riscoe, E., Thrash, C., Bennett, S. E., Tzeng, S.-C. and Maier, C. S. (2011), A freshwater cyanophage whose genome indicates close relationships to photosynthetic marine cyanomyophages. Environmental Microbiology, 13: 1858–1874. doi:10.1111/j.1462-2920.2011.02502.x
Academic Affiliation
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2014-08-22T17:14:40Z (GMT). No. of bitstreams: 2 DreherTheoMicrobiologyFreshwaterCyanophageGenome.pdf: 434118 bytes, checksum: 506728574c5d4a3c06c9f2d72dd849ac (MD5) DreherTheoMicrobiologyFreshwaterCyanophageGenome_SupplementaryMaterial.zip: 387726 bytes, checksum: 410c2dee85d811f60f2551b601d13c6e (MD5) Previous issue date: 2011-05-23
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-08-22T17:14:21Z No. of bitstreams: 2 DreherTheoMicrobiologyFreshwaterCyanophageGenome.pdf: 434118 bytes, checksum: 506728574c5d4a3c06c9f2d72dd849ac (MD5) DreherTheoMicrobiologyFreshwaterCyanophageGenome_SupplementaryMaterial.zip: 387726 bytes, checksum: 410c2dee85d811f60f2551b601d13c6e (MD5)
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-08-22T17:14:40Z (GMT) No. of bitstreams: 2 DreherTheoMicrobiologyFreshwaterCyanophageGenome.pdf: 434118 bytes, checksum: 506728574c5d4a3c06c9f2d72dd849ac (MD5) DreherTheoMicrobiologyFreshwaterCyanophageGenome_SupplementaryMaterial.zip: 387726 bytes, checksum: 410c2dee85d811f60f2551b601d13c6e (MD5)

Relationships

Parents:

This work has no parents.

Last modified Default

Items