Other Scholarly Content

 

Na +/H + Antiport Is Essential for Yersinia pestis Virulence Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/defaults/h702q715z

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by American Society for Microbiology and can be found at:  http://www.asm.org/.

Descriptions

Attribute NameValues
Alternative Title
  • Na[superscript +]/H[superscript +] Antiport Is Essential for Yersinia pestis Virulence
Creator
Abstract
  • Na⁺/H⁺ antiporters are ubiquitous membrane proteins that play a central role in the ion homeostasis of cells. In this study, we examined the possible role of Na⁺/H⁺ antiport in Yersinia pestis virulence and found that Y. pestis strains lacking the major Na⁺/H⁺ antiporters, NhaA and NhaB, are completely attenuated in an in vivo model of plague. The Y. pestis derivative strain lacking the nhaA and nhaB genes showed markedly decreased survival in blood and blood serum ex vivo. Complementation of either nhaA or nhaB in trans restored the survival of the Y. pestis nhaA nhaB double deletion mutant in blood. The nhaA nhaB double deletion mutant also showed inhibited growth in an artificial serum medium, Opti-MEM, and a rich LB-based medium with Na⁺ levels and pH values similar to those for blood. Taken together, these data strongly suggest that intact Na⁺/H⁺ antiport is indispensable for the survival of Y. pestis in the bloodstreams of infected animals and thus might be regarded as a promising noncanonical drug target for infections caused by Y. pestis and possibly for those caused by other blood-borne bacterial pathogens.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Minato, Y., Dibrov, P., Häse, C. C., Ghosh, A., Faulkner, W. J., Lind, E. J., . . . Winogrodzki, J. (2013). Na+/H+ antiport is essential for yersinia pestis virulence. Infection and Immunity, 81(9), 3163-3172. doi:10.1128/IAI.00071-13
Academic Affiliation
Series
Keyword
Rights Statement
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2013-09-18T16:03:43Z (GMT). No. of bitstreams: 1 MinatoYusukeVeterinaryMedicineAntiportEssentialYersinia.pdf: 1082881 bytes, checksum: b68c13bc0c513afff3bbe3b2e604521b (MD5) Previous issue date: 2013-06-17
  • description.provenance : Submitted by Deborah Campbell (deborah.campbell@oregonstate.edu) on 2013-09-18T16:01:11Z No. of bitstreams: 1 MinatoYusukeVeterinaryMedicineAntiportEssentialYersinia.pdf: 1082881 bytes, checksum: b68c13bc0c513afff3bbe3b2e604521b (MD5)
  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-09-18T16:03:43Z (GMT) No. of bitstreams: 1 MinatoYusukeVeterinaryMedicineAntiportEssentialYersinia.pdf: 1082881 bytes, checksum: b68c13bc0c513afff3bbe3b2e604521b (MD5)

Relationships

Parents:

This work has no parents.

Items