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AFLP Analysis of DNA from Dried Hop Cones

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https://ir.library.oregonstate.edu/concern/articles/k643b1895

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  • Hop (Humulus lupulus L.) cones are used extensively in beer brewing to enhance flavor and impart bittering. Verifying hops cultivar identity has traditionally been accomplished by morphological characteristics or a chemical analysis of lupulin glands but these traits may vary according to environmental influences. The objective of this research was to develop an AFLP (amplified fragment length polymorphism) protocol for analyzing DNA extracted from dried hop cones. The DNA was extracted from dried cones of six hop genotypes by a technique published for grapes (Vitis spp). The reagents, MseI primers, and protocol were part of a commercially available kit, while the 6-carboxyfluorescein-labeled EcoRI primers were purchased separately. Eleven primer combinations amplified an average of 546.5 scorable fragments with an average of 49.7 fragments per primer combination. All genotypes were differentiated with the primer combinations studied. Average genetic similarity estimates ranged between 0.956 and 0.995 among the six hop genotypes studied. This research provides the hops industry with a powerful technique to verify accurately hops cultivar identity and purity through an analysis of dried cone DNA.
  • This is the publisher’s final pdf. The published article is copyrighted by the Crop Science Society of America and can be found at: https://www.crops.org/publications/cs
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  • Townsend, M. S., Henning, J. A., & Moore, D. L. (2000). AFLP analysis of DNA from dried hop cones. Crop Science, 40(5), 1383-1386. doi:10.2135/cropsci2000.4051383x
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  • 40
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  • 5
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