Identification of Myosin XI Receptors in Arabidopsis Defines a Distinct Class of Transport Vesicles Public Deposited

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  • To characterize the mechanism through which myosin XI-K attaches to its principal endomembrane cargo, a yeast two-hybrid library of Arabidopsis thaliana cDNAs was screened using the myosin cargo binding domain as bait. This screen identified two previously uncharacterized transmembrane proteins (hereinafter myosin binding proteins or MyoB1/2) that share a myosin binding, conserved domain of unknown function 593 (DUF593). Additional screens revealed that MyoB1/2 also bind myosin XI-1, whereas myosin XI-I interacts with the distantly related MyoB7. The in vivo interactions of MyoB1/2 with myosin XI-K were confirmed by immunoprecipitation and colocalization analyses. In epidermal cells, the yellow fluorescent protein-tagged MyoB1/2 localize to vesicles that traffic in a myosin XI-dependent manner. Similar to myosin XI-K, MyoB1/2 accumulate in the tip-growing domain of elongating root hairs. Gene knockout analysis demonstrated that functional cooperation between myosin XI-K and MyoB proteins is required for proper plant development. Unexpectedly, the MyoB1-containing vesicles did not correspond to brefeldin A-sensitive Golgi and post-Golgi or prevacuolar compartments and did not colocalize with known exocytic or endosomal compartments. Phylogenomic analysis suggests that DUF593 emerged in primitive land plants and founded a multigene family that is conserved in all flowering plants. Collectively, these findings indicate that MyoB are membrane-anchored myosin receptors that define a distinct, plant-specific transport vesicle compartment.
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  • Peremyslov, V. V., Morgun, E. A., Kurth, E. G., Makarova, K. S., Koonin, E. V., & Dolja, V. V. (2013). Identification of myosin XI receptors in arabidopsis defines a distinct class of transport vesicles. The Plant Cell, 25(8), 3022-3038. doi:10.1105/tpc.113.113704
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  • description.provenance : Submitted by Deborah Campbell (deborah.campbell@oregonstate.edu) on 2013-10-29T17:34:10Z No. of bitstreams: 1 PeremyslovValeraVBotanyPlantPathologyIdentificationMyosinXI.pdf: 1747039 bytes, checksum: e207529f2354c551d9d1a35eb97afdfc (MD5)
  • description.provenance : Made available in DSpace on 2013-10-29T17:36:31Z (GMT). No. of bitstreams: 1 PeremyslovValeraVBotanyPlantPathologyIdentificationMyosinXI.pdf: 1747039 bytes, checksum: e207529f2354c551d9d1a35eb97afdfc (MD5) Previous issue date: 2013-08
  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-10-29T17:36:31Z (GMT) No. of bitstreams: 1 PeremyslovValeraVBotanyPlantPathologyIdentificationMyosinXI.pdf: 1747039 bytes, checksum: e207529f2354c551d9d1a35eb97afdfc (MD5)

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