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Identification of Myosin XI Receptors in Arabidopsis Defines a Distinct Class of Transport Vesicles

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  • To characterize the mechanism through which myosin XI-K attaches to its principal endomembrane cargo, a yeast two-hybrid library of Arabidopsis thaliana cDNAs was screened using the myosin cargo binding domain as bait. This screen identified two previously uncharacterized transmembrane proteins (hereinafter myosin binding proteins or MyoB1/2) that share a myosin binding, conserved domain of unknown function 593 (DUF593). Additional screens revealed that MyoB1/2 also bind myosin XI-1, whereas myosin XI-I interacts with the distantly related MyoB7. The in vivo interactions of MyoB1/2 with myosin XI-K were confirmed by immunoprecipitation and colocalization analyses. In epidermal cells, the yellow fluorescent protein-tagged MyoB1/2 localize to vesicles that traffic in a myosin XI-dependent manner. Similar to myosin XI-K, MyoB1/2 accumulate in the tip-growing domain of elongating root hairs. Gene knockout analysis demonstrated that functional cooperation between myosin XI-K and MyoB proteins is required for proper plant development. Unexpectedly, the MyoB1-containing vesicles did not correspond to brefeldin A-sensitive Golgi and post-Golgi or prevacuolar compartments and did not colocalize with known exocytic or endosomal compartments. Phylogenomic analysis suggests that DUF593 emerged in primitive land plants and founded a multigene family that is conserved in all flowering plants. Collectively, these findings indicate that MyoB are membrane-anchored myosin receptors that define a distinct, plant-specific transport vesicle compartment.
  • Keywords: Protein body formation, Endoplasmic reticulum motility, Membrane, Brefeldin-A, trafficking, Golgi stacks, Rppt hair, F actin organization, Terminal domain, Plant Cells
  • Keywords: Protein body formation, Endoplasmic reticulum motility, Membrane, Brefeldin-A, trafficking, Golgi stacks, Rppt hair, F actin organization, Terminal domain, Plant Cells
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  • Peremyslov, V. V., Morgun, E. A., Kurth, E. G., Makarova, K. S., Koonin, E. V., & Dolja, V. V. (2013). Identification of myosin XI receptors in arabidopsis defines a distinct class of transport vesicles. The Plant Cell, 25(8), 3022-3038. doi:10.1105/tpc.113.113704
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  • 25
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  • 8
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  • The initial stage of this work was supported by National Institutes of Health Award R01 GM087658 to V.V.D. K.S.M and E.V.K. are supported by intramural funds of the U.S. Department of Health and Human Services (to the National Library of Medicine).
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