Understanding the impact of orientation on gene expression of lux operon in pKN800 transformation into Escherichia coli DH5α Public Deposited

http://ir.library.oregonstate.edu/concern/defaults/n583z028r

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  • Isolate and purify a pKN800 plasmid from Escherichia coli. Using restriction mapping determine the orientation of the Luciferase (lux) operon with respect to the plasmid backbone. Then transform the plasmid into E. coli strain DH5α to analyze the expression of the lux operon and the efficiency of transformation.
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  • description.provenance : Approved for entry into archive by Steven Van Tuyl(steve.vantuyl@oregonstate.edu) on 2017-05-01T21:15:49Z (GMT) No. of bitstreams: 1 Impact of Gene Orientation of Lux Operon Expression.pdf: 789768 bytes, checksum: 6b29120c2c264535ef9214428deb34b4 (MD5)
  • description.provenance : Made available in DSpace on 2017-05-01T21:15:50Z (GMT). No. of bitstreams: 1 Impact of Gene Orientation of Lux Operon Expression.pdf: 789768 bytes, checksum: 6b29120c2c264535ef9214428deb34b4 (MD5)
  • description.provenance : Submitted by Darian Seim (seimd@oregonstate.edu) on 2017-04-11T01:17:11Z No. of bitstreams: 1 Impact of Gene Orientation of Lux Operon Expression.pdf: 789768 bytes, checksum: 6b29120c2c264535ef9214428deb34b4 (MD5)

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