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Understanding the impact of orientation on gene expression of lux operon in pKN800 transformation into Escherichia coli DH5α Öffentlichkeit Deposited

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https://ir.library.oregonstate.edu/concern/defaults/n583z028r

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  • Isolate and purify a pKN800 plasmid from Escherichia coli. Using restriction mapping determine the orientation of the Luciferase (lux) operon with respect to the plasmid backbone. Then transform the plasmid into E. coli strain DH5α to analyze the expression of the lux operon and the efficiency of transformation.
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