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Virus-Derived Gene Expression and RNA Interference Vector for Grapevine Public Deposited

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  • The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction of desired traits into grapevine without heritable modifications to the genome. This vector expresses recombinant proteins in the phloem tissue that is involved in sugar transport throughout the plant, from leaves to roots to berries. Furthermore, the vector provides a powerful RNA interference (RNAi) capability of regulating the expression of endogenous genes via virus-induced gene-silencing (VIGS) technology. Additional advantages of this vector include superb genetic capacity and stability, as well as the swiftness of technology implementation. The most significant applications of the viral vector include functional genomics of the grapevine and disease control via RNAi-enabled vaccination against pathogens or invertebrate pests.
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  • Kurth, E. G., Peremyslov, V. V., Prokhnevsky, A. I., Kasschau, K. D., Miller, M., Carrington, J. C., & Dolja, V. V. (2012). Virus-derived gene expression and RNA interference vector for grapevine. Journal of Virology, 86(11), 6002-6009. doi: 10.1128/JVI.00436-12
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  • This work was supported by contract 2010-744 from Vinoculate, Inc., Soledad, CA, a subcontract from USDA-NIFA (award no. 2009-04401), and BARD award no. IS-4314-10C to V.V.D. The work in J.C.C. lab is supported by NSF award MCB-1231726 and NIH award AI43288.
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  • description.provenance : Made available in DSpace on 2013-03-20T20:30:48Z (GMT). No. of bitstreams: 1 DoljaValerianBotanyPlantPathologyVirusDerivedGene.pdf: 3084824 bytes, checksum: 5a14aba0da556c42711dcf0343b66416 (MD5) Previous issue date: 2012-06
  • description.provenance : Approved for entry into archive by Deanne Bruner(deanne.bruner@oregonstate.edu) on 2013-03-20T20:30:48Z (GMT) No. of bitstreams: 1 DoljaValerianBotanyPlantPathologyVirusDerivedGene.pdf: 3084824 bytes, checksum: 5a14aba0da556c42711dcf0343b66416 (MD5)
  • description.provenance : Submitted by Deanne Bruner (deanne.bruner@oregonstate.edu) on 2013-03-20T20:30:12Z No. of bitstreams: 1 DoljaValerianBotanyPlantPathologyVirusDerivedGene.pdf: 3084824 bytes, checksum: 5a14aba0da556c42711dcf0343b66416 (MD5)

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