Ribonucleotide Reductase Association with Mammalian Liver Mitochondria Public Deposited

http://ir.library.oregonstate.edu/concern/defaults/ns064b62t

This research was originally published in The Journal of Biological Chemistry. Chimploy, K., Song, S., Wheeler, L. J., & Mathews, C. K. Ribonucleotide reductase association with mammalian liver mitochondria. 2013. 288(18), 13145-13155. © the American Society for Biochemistry and Molecular Biology

This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Society for Biochemistry and Molecular Biology and can be found at:  http://www.jbc.org/.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Deoxyribonucleoside triphosphate pools in mammalian mitochondria are highly asymmetric, and this asymmetry probably contributes toward the elevated mutation rate for the mitochondrial genome as compared with the nuclear genome. To understand this asymmetry, we must identify pathways for synthesis and accumulation of dNTPs within mitochondria. We have identified ribonucleotide reductase activity specifically associated with mammalian tissue mitochondria. Examination of immunoprecipitated proteins by mass spectrometry revealed R1, the large RNR subunit, in purified mitochondria. Significant enzymatic and immunological activity was seen in rat liver mitochondrial nucleoids, isolated as described by Wang, Y., and Bogenhagen, D. F. (2006) J. Biol. Chem. 281, 25791–25802. Moreover, incubation of respiring rat liver mitochondria with [¹⁴C]cytidine diphosphate leads to acccumulation of radiolabeled deoxycytidine and thymidine nucleotides within the mitochondria. Comparable results were seen with [¹⁴C]guanosine diphosphate. Ribonucleotide reduction within the mitochondrion, as well as outside the organelle, needs to be considered as a possibly significant contributor to mitochondrial dNTP pools.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Chimploy, K., Song, S., Wheeler, L. J., & Mathews, C. K. (2013). Ribonucleotide reductase association with mammalian liver mitochondria. The Journal of Biological Chemistry, 288(18), 13145-13155. doi:10.1074/jbc.M113.461111
Academic Affiliation
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items