Protein Adducts of Aldehydic Lipid Peroxidation Products: Identification and Characterization of Protein Adducts Using an Aldehyde/Keto Reactive Probe in Combination with Mass Spectrometry Public Deposited

http://ir.library.oregonstate.edu/concern/defaults/p5547s088

This is an author's manuscript, as accepted by the publisher. The published chapter 16 is copyrighted by Elsevier.

Descriptions

Attribute NameValues
Alternative Title
Creator
Abstract or Summary
  • This chapter describes a mass spectrometry-based strategy that facilitates the unambiguous identification and characterization of proteins modified by lipid peroxidation-derived 2-alkenals. The approach employs a biotinylated hydroxyl amine derivative as an aldehyde/keto reactive probe in conjunction with selective enrichment and tandem mass spectrometric analysis. Methodological details are given for model studies involving a distinct protein and 4-hydroxy-2-nonenal (HNE). The method was also evaluated for an exposure study of a cell culture system with HNE that yielded the major protein targets of HNE in human monocytic THP-1 cells. The application of the approach to complex biological systems is demonstrated for the identification and characterization of endogenous protein targets of aldehydic lipid peroxidation products present in cardiac mitochondria.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Maier, C. S., Chavez, J., Wang, J., & Wu, J. (2010). Protein adducts of aldehydic lipid peroxidation products: identification and characterization of protein adducts using an aldehyde/keto-reactive probe in combination with mass spectrometry. Methods in Enzymology, 473, 305-330. doi:10.1016/S0076-6879(10)73016-0
Academic Affiliation
Series
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-11-14T00:19:40Z No. of bitstreams: 1 MaierClaudiaChemistryProteinAdductsAldehydic.pdf: 493212 bytes, checksum: 8d5277f44c79b9ac05f613b5d2efbae8 (MD5)
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-11-14T00:19:59Z (GMT) No. of bitstreams: 1 MaierClaudiaChemistryProteinAdductsAldehydic.pdf: 493212 bytes, checksum: 8d5277f44c79b9ac05f613b5d2efbae8 (MD5)
  • description.provenance : Made available in DSpace on 2014-11-14T00:19:59Z (GMT). No. of bitstreams: 1 MaierClaudiaChemistryProteinAdductsAldehydic.pdf: 493212 bytes, checksum: 8d5277f44c79b9ac05f613b5d2efbae8 (MD5) Previous issue date: 2010

Relationships

In Administrative Set:
Last modified: 07/11/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items