Capsaicin represses transcriptional activity of β-catenin in human colorectal cancer cells Public Deposited

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This is the author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier and can be found at:  http://www.journals.elsevier.com/the-journal-of-nutritional-biochemistry/.

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  • Capsaicin is a pungent ingredient in chili red peppers and has been linked to suppression of growth in various cancer cells. However, the underlying mechanism(s) by which capsaicin induces growth arrest and apoptosis of cancer cells is not completely understood. In the present study, we investigated whether capsaicin alters β-catenin-dependent signaling in human colorectal cancer cells in vitro. Exposure of SW480, LoVo, and HCT-116 cells to capsaicin suppressed cell proliferation. Transient transfection with a β-catenin/T-cell factor (TCF)-responsive reporter indicated that capsaicin suppressed the transcriptional activity of β-catenin/TCF. Capsaicin treatment resulted in a decrease of intracellular β-catenin levels and a reduction of transcripts from the β-catenin gene (CTNNB1). These results were confirmed by a reduced luciferase reporter activity driven by promoter-reporter construct containing the promoter region of the Catnb gene. In addition, capsaicin destabilized β-catenin through enhancement of proteosomal-dependent degradation. Western blot and immunoprecipitation studies indicated that capsaicin treatment suppressed TCF-4 expression and disrupted the interaction of TCF-4 and β-catenin. This study identifies a role for the β-catenin/TCF-dependent pathway that potentially contributes to the anti-cancer activity of capsaicin in human colorectal cancer cells.
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  • Lee, S., Richardson, R. L., Dashwood, R. H., & Baek, S. J. (2012). Capsaicin represses transcriptional activity of β-catenin in human colorectal cancer cells. The Journal of Nutritional Biochemistry, 23(6), 646.
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  • description.provenance : Approved for entry into archive by Deanne Bruner(deanne.bruner@oregonstate.edu) on 2013-03-15T23:35:07Z (GMT) No. of bitstreams: 2 DashwoodRoderickEnvironmentalMolecularToxicologyCapsaicinRepressesTranscriptionalFigures1-7(PowerPoint).pptx: 872282 bytes, checksum: 43d590e14a5a6ae34ff5346eebe8c078 (MD5) DashwoodRoderickEnvironmentalMolecularToxicologyCapsaicinRepressesTranscriptional .pdf: 438953 bytes, checksum: 19e6dde61e11ae45b5100033387e63d9 (MD5)
  • description.provenance : Made available in DSpace on 2013-03-15T23:35:07Z (GMT). No. of bitstreams: 2 DashwoodRoderickEnvironmentalMolecularToxicologyCapsaicinRepressesTranscriptionalFigures1-7(PowerPoint).pptx: 872282 bytes, checksum: 43d590e14a5a6ae34ff5346eebe8c078 (MD5) DashwoodRoderickEnvironmentalMolecularToxicologyCapsaicinRepressesTranscriptional .pdf: 438953 bytes, checksum: 19e6dde61e11ae45b5100033387e63d9 (MD5) Previous issue date: 2012-06
  • description.provenance : Submitted by Deanne Bruner (deanne.bruner@oregonstate.edu) on 2013-03-15T23:34:01Z No. of bitstreams: 2 DashwoodRoderickEnvironmentalMolecularToxicologyCapsaicinRepressesTranscriptionalFigures1-7(PowerPoint).pptx: 872282 bytes, checksum: 43d590e14a5a6ae34ff5346eebe8c078 (MD5) DashwoodRoderickEnvironmentalMolecularToxicologyCapsaicinRepressesTranscriptional .pdf: 438953 bytes, checksum: 19e6dde61e11ae45b5100033387e63d9 (MD5)

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