Transgenic expression of Walleye dermal sarcoma virus rv-cyclin gene in zebrafish and its protective effect on liver-tumor development after carcinogen treatment Public Deposited

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This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer and can be found at:  http://link.springer.com/journal/10126. In the published article, the author, Guor Muor Her, has been added and the word protective has been changed to suppressive in the title.

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  • A retrovirus homologue gene of cellular cyclin D₁, walleye dermal sarcoma virus rv-cyclin gene (orf A or rv-cyclin), was expressed in the livers of zebrafish under the control of liver fatty-acid binding protein (lfabp) promoter. To prevent possible fatality caused by over-expression of the oncogene, the GAL4/UAS system was used to maintain the transgenic lines. Thus, both GAL4-activator, Tg(lfabp:GAL4), and UAS-effector, Tg(UAS:rvcyclin), lines were generated and the rv-cyclin gene was activated in the liver after crossing these two lines. Since no obvious neoplasial phenotypes were observed in the double-transgenic line, cancer susceptibility of the transgenic fish expressing rv-cyclin was tested by carcinogen treatment. Unexpectedly, transgenic fish expressing rv-cyclin gene (rvcyclin+) seemed to be more resistant to the carcinogen than were siblings not expressing this gene (rvcyclin–). Lower incidences of multiple and malignant liver tumors were observed in rvcyclin+ than in rvcyclin– fish, and the liver tumors in the rvcyclin+ group appeared later and less severe. These results suggest that expression of rv-cyclin protects the fish liver from carcinogen damage and delays onset of malignancy. This observation—from a transgenic fish model—may be relevant to studies of liver-cancer inhibition and regression.
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  • Zhan, H., Spitsbergen, J. M., Qing, W., Wu, Y. L., Paul, T. A., Casey, J. W., ... & Gong, Z. (2010). Transgenic expression of walleye dermal sarcoma virus rv-cyclin gene in zebrafish and its suppressive effect on liver tumor development after carcinogen treatment. Marine Biotechnology, 12(6), 640-649. doi:10.1007/s10126-009-9251-9
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  • description.provenance : Made available in DSpace on 2014-08-06T20:00:34Z (GMT). No. of bitstreams: 1 SpitsbergenJanMicrobiologyTransgenicExpressionWalleye.pdf: 623123 bytes, checksum: 33bfba180fcbcd43034810b2a53d0120 (MD5) Previous issue date: 2010-11
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-08-06T20:00:01Z No. of bitstreams: 1 SpitsbergenJanMicrobiologyTransgenicExpressionWalleye.pdf: 623123 bytes, checksum: 33bfba180fcbcd43034810b2a53d0120 (MD5)
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-08-06T20:00:34Z (GMT) No. of bitstreams: 1 SpitsbergenJanMicrobiologyTransgenicExpressionWalleye.pdf: 623123 bytes, checksum: 33bfba180fcbcd43034810b2a53d0120 (MD5)

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