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Transgenic expression of Walleye dermal sarcoma virus rv-cyclin gene in zebrafish and its protective effect on liver-tumor development after carcinogen treatment

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  • A retrovirus homologue gene of cellular cyclin D₁, walleye dermal sarcoma virus rv-cyclin gene (orf A or rv-cyclin), was expressed in the livers of zebrafish under the control of liver fatty-acid binding protein (lfabp) promoter. To prevent possible fatality caused by over-expression of the oncogene, the GAL4/UAS system was used to maintain the transgenic lines. Thus, both GAL4-activator, Tg(lfabp:GAL4), and UAS-effector, Tg(UAS:rvcyclin), lines were generated and the rv-cyclin gene was activated in the liver after crossing these two lines. Since no obvious neoplasial phenotypes were observed in the double-transgenic line, cancer susceptibility of the transgenic fish expressing rv-cyclin was tested by carcinogen treatment. Unexpectedly, transgenic fish expressing rv-cyclin gene (rvcyclin+) seemed to be more resistant to the carcinogen than were siblings not expressing this gene (rvcyclin–). Lower incidences of multiple and malignant liver tumors were observed in rvcyclin+ than in rvcyclin– fish, and the liver tumors in the rvcyclin+ group appeared later and less severe. These results suggest that expression of rv-cyclin protects the fish liver from carcinogen damage and delays onset of malignancy. This observation—from a transgenic fish model—may be relevant to studies of liver-cancer inhibition and regression.
  • Keywords: Transgenic fish, Carcinogen, Liver neoplasia, Tumor regression, Walleye dermal sarcoma virus rv-cyclin gene
  • Keywords: Transgenic fish, Carcinogen, Liver neoplasia, Tumor regression, Walleye dermal sarcoma virus rv-cyclin gene
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  • Zhan, H., Spitsbergen, J. M., Qing, W., Wu, Y. L., Paul, T. A., Casey, J. W., ... & Gong, Z. (2010). Transgenic expression of walleye dermal sarcoma virus rv-cyclin gene in zebrafish and its suppressive effect on liver tumor development after carcinogen treatment. Marine Biotechnology, 12(6), 640-649. doi:10.1007/s10126-009-9251-9
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  • 12
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  • 6
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  • Our research was supported by grants from the Biomedical Research Council (BMRC) of Singapore.
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