pHrodo™ Green E. coli Bioparticles are a Viable Method for Quantifying Engulfment Activity in Anoplopoma fimbria (Sablefish) Phagocytes

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Citeable URL: https://ir.library.oregonstate.edu/concern/defaults/th83m5759

Descriptions

Attribute Name	Values
Creator	Mendoza, Anabel A.
Abstract	Sablefish is a highly sought after seafood delicacy for which aquaculture of this species has recently been developed. By understanding the immunological functions in sablefish, our goal was to develop a mammalian fluorescent bioparticle assay that can viably analyze the efficiency of phagocytosis, an important function of the immune system. Our results showed that pHrodo fluorescent E. coli bioparticles read after 3 hours are the superior method to quantitatively assess engulfment activity in Sablefish phagocytes. This assay supported a larger study in determining the effects of herbal supplements on the immunological function of sablefish when exposed to lethal concentrations of Aeromonas salmonicida.
Contributor	Arkoosh, Mary R.
License	Attribution-NonCommercial 4.0 (CC BY-NC 4.0)
Resource Type	Poster
Date Issued	2019-09-05
Degree Level	Bachelor's
Degree Name	Bachelor of Science (B.S.)
Degree Field	BioHealth Sciences
Advisor	Schubiger, Carla B.
Academic Affiliation	Biomedical Sciences
Rights Statement	In Copyright
Publisher	Oregon State University
Peer Reviewed	No
Language	English [eng]
Additional Information	OSU College of Science SURE Program Carlson College of Veterinary Medicine Summer Undergraduate Research Program

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