Other Scholarly Content

 

pHrodo™ Green E. coli Bioparticles are a Viable Method for Quantifying Engulfment Activity in Anoplopoma fimbria (Sablefish) Phagocytes Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/defaults/th83m5759

Descriptions

Attribute NameValues
Creator
Abstract
  • Sablefish is a highly sought after seafood delicacy for which aquaculture of this species has recently been developed. By understanding the immunological functions in sablefish, our goal was to develop a mammalian fluorescent bioparticle assay that can viably analyze the efficiency of phagocytosis, an important function of the immune system. Our results showed that pHrodo fluorescent E. coli bioparticles read after 3 hours are the superior method to quantitatively assess engulfment activity in Sablefish phagocytes. This assay supported a larger study in determining the effects of herbal supplements on the immunological function of sablefish when exposed to lethal concentrations of Aeromonas salmonicida.
Contributor
License
Resource Type
Date Issued
Degree Level
Degree Name
Degree Field
Advisor
Academic Affiliation
Rights Statement
Publisher
Peer Reviewed
Language
Additional Information
  • OSU College of Science SURE Program
  • Carlson College of Veterinary Medicine Summer Undergraduate Research Program

Relationships

Parents:

This work has no parents.

Items