Effects of megagametophyte removal on DNA yield and early seedling growth in coastal Douglas-fir. Public Deposited

http://ir.library.oregonstate.edu/concern/defaults/wm117p48w

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  • Stratified seeds of coastal Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco var. menziesii) were germinated, sown in soil, and seed coats and megagametophytes were removed at various stages of early seedling development. Yield and quality of DNA extracted from the megagametophytes were related to several morphological traits of the seedlings after 2 months of growth in a controlled environment chamber. Regression and analysis of variance demonstrated nonlinear associations between stage of megagametophyte removal and seedling size traits, DNA yield and quality, and RNA presence. Megagametophyte removal when cotyledons had extended one-quarter of their length (about 4 mm) outside the seed coat (our stage 4) resulted in sufficient DNA for construction of saturated PCR (polymerase chain reaction) based genome maps and had little effect on seedling development.
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  • Krutovskii, K.V., S.S. Vollmer, F.C. Sorensen, W.T. Adams, and S.H. Strauss. 1997. Effects of megagametophyte removal on DNA yield and early seedling growth in coastal Douglas-fir. Canadian Journal of Forest Research 27(6): 964-968.
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  • description.provenance : Made available in DSpace on 2010-02-03T23:43:25Z (GMT). No. of bitstreams: 1 Krutovskii_etal97.pdf: 87455 bytes, checksum: 3ff03b2fa5c4636b181708ead275d520 (MD5) Previous issue date: 1997-06
  • description.provenance : Approved for entry into archive by Caryn Davis(caryn.davis@oregonstate.edu) on 2010-02-03T23:43:25Z (GMT) No. of bitstreams: 1 Krutovskii_etal97.pdf: 87455 bytes, checksum: 3ff03b2fa5c4636b181708ead275d520 (MD5)
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