Article
 

The mitochondrial genomes of Euphausia pacifica and Thysanoessa raschii sequenced using 454 next-generation sequencing, with a phylogenetic analysis of their position in the Malacostracan family tree

Public Deposited

Contenu téléchargeable

Télécharger le fichier PDF
https://ir.library.oregonstate.edu/concern/articles/zk51vh359

Descriptions

Attribute NameValues
Creator
Abstract
  • Euphausiid krill play a critical role in coastal and oceanic food webs, linking primary producers to upper trophic levels. In addition, some species support commercial fisheries worldwide. Despite their ecological importance, the genetics of these important species remain poorly described. To improve our understanding of the genetics of these ecological links, we sequenced the mitochondrial genomes of two species of North Pacific krill, Euphausia pacifica and Thysanoessa raschii, using long-range PCR and 454 GS Junior next-generation sequencing technology. The E. pacifica mitogenome (14,692 + base pairs (bp)) encodes 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, and at least 22 transfer RNA (tRNA) genes. The T. raschii mitogenome (14,240 + bp) encodes 13 PCGs, two rRNA genes, and at least 19 tRNA genes. The gene order in both species is similar to that of E. superba. Comparisons between Bering Sea and Yellow Sea E. pacifica revealed a total of 644 variable sites. The most variable protein-coding gene were atp8 (7.55 %, 12 of 159 sites variable), nad4 (6.35 %, 85 variable sites) and nad6 (6.32 %, 33 variable sites). Phylogenetic analyses to assess the phylogenetic position of the Euphausiacea, using the concatenated nucleic acid sequences of E. pacifica and T. raschii along with 46 previously published malacostracan mitogenomes, support the monophyly of the order Decapoda and indicate that the Euphausiacea share a common ancestor with the Decapoda. Future research should utilize this sequence data to explore the population genetics and molecular ecology of these species.
  • Keywords: Malacostraca, Euphausiacea, Mitochondrial genome, mtDNA, Phylogeny
  • Keywords: Malacostraca, Euphausiacea, Mitochondrial genome, mtDNA, Phylogeny
Resource Type
DOI
Date Available
Date Issued
Citation
  • Johansson, M., Sremba, A., Feinberg, L., Banks, M., & Peterson, W. (2012). The mitochondrial genomes of euphausia pacifica and thysanoessa raschii sequenced using 454 next-generation sequencing, with a phylogenetic analysis of their position in the malacostracan family tree. MOLECULAR BIOLOGY REPORTS, 39(9), 9009-9021. doi: 10.1007/s11033-012-1772-z
Journal Title
Journal Volume
  • 39
Journal Issue/Number
  • 9
Non-Academic Affiliation
Déclaration de droits
Funding Statement (additional comments about funding)
  • This work was supported by a NOAA award to CIMRS (Award No. NA06NMF4550286).
Publisher
Peer Reviewed
Language
Replaces

Des relations

Parents:

This work has no parents.

Dans Collection:

Articles

La vignette Titre Date de téléchargement Visibilité actes
file details JohanssonMattiasHMSCMitochondrialGenomesEuphausia.pdf 2017-07-07 Public Télécharger