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Measuring the Lipase Activity of Rabbit Gastric Enzyme for In-Vitro Digestion of Human Milk

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  • In vitro digestion is the process of simulating digestion outside the body. In vitro digestion models are rapid, reproducible, and cost effective methods to research human digestion when trials in in vivo models are not suitable. In vitro digestion is conducted based on in vivo physiological factors such as pH, temperature, chemical composition and enzymes and is performed in multiple stages to mimic in vivo digestion in a population. To accurately simulate a human population, these parameters must be reflected in the simulated in vitro digestion. One key digestive enzyme used in in vitro digestion is rabbit gastric extract (RGE) due to its similarity to human gastric lipase. To determine the amount of lipase from RGE to add to our in vitro digestion to properly simulate human gastric lipase levels, we measured the gastric lipase enzymatic activity of RGE using a lipase enzyme activity assay. We found that the average RGE lipase enzyme activity was 7.0 units per milligram of RGE, with a standard deviation of 1.4 units per milligram. Using these values, we can accurately add RGE during our in vitro digestions to mimic in vivo human digestions.
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