Graduate Thesis Or Dissertation
 

The characterization of the MFO system in rainbow trout fed cruciferous vegetables

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  • Several compounds have been shown to induce the mixed function oxidase enzyme system of animals, and also to inhibit tumor formation. Included in this category are several vegetables and isolated vegetable components from the Cruciferae family. In this study rainbow trout were fed blanched freeze-dried cauliflower, broccoli, or Brussel sprouts at the 20% supplemental level for two months, or 500 ppm benzyl or phenethyl isothiocyanate. At this time the livers were removed and hepatic microsomal enzymes were studied. This treatment did not cause any increases in the metabolism of p-nitroanisole or benzo(a)pyrene and cytochrome P-450 levels were not elevated. On the contrary, benzo(a)pyrene monooxygenase, 7-ethoxycoumarin 0-dealkylase, resorufin 0-dealkylase, p-nitroanisole 0-demethylase, and cytochrome P-450 levels were increased in trout treated orally and intraperitoneally with β-napthoflavone. For all parameters studied, a greater increase in activity was seen in the trout treated intraperitoneally. The vegetable and isothiocyanate supplemented diets also did not inhibit tumor formation in trout challenged with 20 ppb aflatoxin B₁ for one month. A study with rats showed that both blanched freeze-dried cauliflower and air-dried cauliflower cause significant increases in p-nitroanisole 0-demethylase activity and benzo(a)pyrene monooxygenase activity and increased cytochrome P-450 levels in rat hepatic microsomes when fed at the 20% supplemental level for 25 days. It was concluded that the null effects seen with the trout fed the vegetables and isothiocyanates were not due to the heat treatment of the vegetables since rats fed blanched freeze-dried cauliflower had significant increases in enzymatic activity and increased cytochrome P-450 levels over controls. The results of the β-napthoflavone experiment verified the validity and reliability of the experimental assays and conditions and showed that trout hepatic microsomal enzymes can be induced by orally administered compounds.
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