Graduate Thesis Or Dissertation
 

Certain chemical and immunological properties of the endotoxin from Vibrio anguillarum

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  • The Boivin agent from Vibrio anguillarum was extracted with the use of 0.25M trichloroacetic acid (TCA). Chemical analysis revealed that the endotoxin contained approximately 35.1% protein, 18.1% carbohydrate, 39.3% lipid, 1.8% glucosamine and evidence of ethanolamine. Sixteen different amino acids made up the protein. The aliphatic amino acid concentration was 41.6%. The acidic amino acid content was higher than the basic amino acid content (25.8% vs. 14.4%) and glutamic and aspartic acid were the predominant amino acids. The carbohydrate fraction was composed of fucose (7.3%), arabinose (1.2%), deoxyglucose (tenative identification) (4.2%), galactose (0.8%), glucose (2.1%), and an unidentified sugar (2.5%). Injection of the endotoxin into mice resulted in an LD₅₀ value of 794 μg. When injected intramuscularly into juvenile chinook salmon, the endotoxin caused a zone of hemorrhaging at the site of injection. Upon prolonged storage of the endotoxin, this activity was lost. When incorporated into the salmonids diet or injected intraperitoneally, the endotoxin protected against vibriosis. A higher degree of protection was obtained by injection than by feeding of the endotoxin. No anti-V. anguillarum agglutinating antibodies were evident in the serum of juvenile chinook salmon fed the endotoxin or whole cell vaccine. Fish receiving the endotoxin by intraperitoneal injection produced anti-V. anguillarum agglutinating serum antibodies.
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