Graduate Thesis Or Dissertation
 

The effect of electrical current on bacteria on beef carcasses, on agar media and in suspensions

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  • Electrical stimulation of beef carcasses (n=18) did not cause a significant reduction in microbial population on three different sampling positions immediately following slaughter. In constrast, significant microbial reduction (P > 0.05) was found at position 2 (muscle above aitch bone), but not at position 1 (inside of neck) or position 3 (fat on outside of round) after 72 h. of chilling storage. Nina bacterial species from eight different genera were inoculated on three different agar media which varied in electrical conductivity. Electrical stimulation of these media caused a reduction in recoveries of microorganisms under various voltage and time treatments. Spore-forming bacteria were the most resistant to the electrical treatment. Among the non-spore-formers, gram negative bacteria were more resistant to electrical treatment than gram positive bacteria. Also, microorganisms inoculated on the lower resistance medium A revealed greater reduction in the recoveries than that of the other media with higher resistance. A five log number reduction (99.999%) in E. coli, P. outrifaciens, and P. fragi was found in 0.85% saline and phosphate buffered saline after a 30 V, 5 min. treatment, but little change in count was detected in o.1% aqueous peptone or 0.25 M sucrose solution.
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