Transformation of alfalfa using Agrobacterium tumefaciens Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/05741w06t

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Agrobacterium tumefaciens, the causative agent of crown gall disease, does not readily infect commercial varieties of alfalfa (Medicago sativa). Cloned virulence genes from the megaplasmid pTiBo542 have been shown to enhance Agrobacterium host range and infectivity on dicotyledonous plants. To obtain a gene transfer system for commercial alfalfa cultivars, an Agrobacterium strain containing a disarmed T-DNA, a cloned virG gene from pTiBo542, and an octapine Ti plasmid TiA6NC was constructed. This strain, A348 (pToK9, pGA472), was used to inoculate excised petioles of the alfalfa cultivar "Gladiator. Neomycin resistant callus tissue grew readily on 100 p.1/mIkanamycin and on appropriate media, leaf and embryo development was induced. DNA hybridization analysis of the transformed plant genomic DNA isolated from callus tissue confirmed the presence of the npt gene. VirG enhanced A. tumefaciens strains can thus be used in foreign gene transfer and expression in commercial cultivars of alfalfa.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 24-bit Color) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-07-16T18:43:02Z (GMT) No. of bitstreams: 1 GelfordIngaL1989.pdf: 1316637 bytes, checksum: e9624260f6eb39f091631cced2e6e54d (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-05-29T16:11:59Z (GMT) No. of bitstreams: 1 GelfordIngaL1989.pdf: 1316637 bytes, checksum: e9624260f6eb39f091631cced2e6e54d (MD5)
  • description.provenance : Submitted by Kaylee Patterson (kdpscanner@gmail.com) on 2013-05-23T20:27:01Z No. of bitstreams: 1 GelfordIngaL1989.pdf: 1316637 bytes, checksum: e9624260f6eb39f091631cced2e6e54d (MD5)
  • description.provenance : Made available in DSpace on 2013-07-16T18:43:02Z (GMT). No. of bitstreams: 1 GelfordIngaL1989.pdf: 1316637 bytes, checksum: e9624260f6eb39f091631cced2e6e54d (MD5) Previous issue date: 1988-06-20

Relationships

In Administrative Set:
Last modified: 08/16/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items