Graduate Thesis Or Dissertation
 

Isoenzyme polymorphism in provenances of Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco var. menziesii)

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/0g354j74r

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  • Genetic variability in natural populations of Douglas-fir was studied at molecular level by investigating the variations of some primary gene products, i. e. enzymes. Using the techniques of disc gel electrophoresis and biochemical staining, the isoenzyme patterns of leucine aminopeptidase, esterase and glutamate oxaloacetate transaminase are characterized in young seedlings at the stage of completely elongated cotyledons. Samples of 107 seedlots were collected from nine provenances located at Vancouver Island of Canada, Washington, Oregon and California. The segregations of isoenzymic phenotypes or genotypes at four polymorphic loci are, in most cases, in accordance with the Hardy-Weinberg expectations except in those provenances from high elevations and from the northern or southern species margins. Geographic variations of frequencies of most alleles are significant among those provenances examined, but appear to be rather complex. By contrast, the variation patterns in level of genic heterozygosity exhibited a certain degree of geographic uniformity or clinal change except that at EST-B locus. The combined effect of gene flow and some form of balancing selection was considered as the probable mechanism in maintaining uniformity or clinal variation of isoenzymic heterozygosity. Regression analysis, principal component method and correlation approach using both the original variables and variables generated as principal component. functions, were used to relate allelic variability of isoenzymes and environmental factors. The significance of adaptation through the selective importance of three enzyme systems was discussed. Genetic differentiation in terms of genetic identity and genetic distance between provenances of Douglas-fir is more or less in parallel to the geographic distance of locations. The parallelism between the level of genic heterozygosity and the growth potential or adaptability for survival of trees of different origins was noted. An approach through a multidimensional study involving functional properties and inheritance patterns of isoenzymes, and environmental modulations was suggested as a possibility to complement genetic improvement programs in Douglas-fir.
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