Investigation of cis-acting RNA element role in bovine viral diarrhea virus replication Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/0k225d73h

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Bovine viral diarrhea virus (BVDV) is an enveloped, single-stranded positive-sense RNA virus. The BVDV genome is about 12.5 kb long and consists of a single open reading frame (ORF) flanked by untranslated regions at the 5' and 3' ends (5'-UTR and 3'- UTR, respectively). To investigate the role of cis-sequences of UTRs during BVDV replication, we designed four peptide-conjugated phosphorodiamidate morpholino oligomers(PPMO) that are complementary to nucleotides (nt) 10-33 (BVDV-10), 258-281 (BVDV- 258), 375-398 (BVDV-375), and 12503-12526 (BVDV-12503) of the BVDV genome. PPMO with sequences targeting nt 258-281 (BVDV-258), 375-398 (BVDV-375), and 12503-12526 (BVDV-12503) of the BVDV genome completely blocked BVDV replication in vitro, while the PPMO with sequence complementary to nt 10-33 (BVDV-10) did not have observable inhibition against BVDV virus replication. In addition, BVDV-375 and BVDV-12503 were able to inhibit virus replication at the viral RNA synthesis level. Our study demonstrated that the cis-sequences nt 258-281 and 375-398 in the 5’-UTR and nt 12503-12526 in the 3’-UTR are important for BVDV replication, while the cis-sequence nt 10-33 may not play a significant role during virus replication.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Non-Academic Affiliation
Keyword
Subject
Rights Statement
Language
Replaces
Additional Information
  • description.provenance : Submitted by David Ly (lyd@onid.orst.edu) on 2009-05-27T01:06:43Z No. of bitstreams: 1 thesis-finalPDF.pdf: 828574 bytes, checksum: 61b41629aa2e87b3f5c7e3b1b4f83645 (MD5)
  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2009-06-04T21:53:22Z (GMT) No. of bitstreams: 1 thesis-finalPDF.pdf: 828574 bytes, checksum: 61b41629aa2e87b3f5c7e3b1b4f83645 (MD5)
  • description.provenance : Made available in DSpace on 2009-06-10T17:45:54Z (GMT). No. of bitstreams: 1 thesis-finalPDF.pdf: 828574 bytes, checksum: 61b41629aa2e87b3f5c7e3b1b4f83645 (MD5)
  • description.provenance : Approved for entry into archive by Linda Kathman(linda.kathman@oregonstate.edu) on 2009-06-10T17:45:54Z (GMT) No. of bitstreams: 1 thesis-finalPDF.pdf: 828574 bytes, checksum: 61b41629aa2e87b3f5c7e3b1b4f83645 (MD5)

Relationships

In Administrative Set:
Last modified: 08/08/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items