Graduate Thesis Or Dissertation
 

Bioavailability of vitamin B-6 from test foods and metabolism of vitamin B-6 in men receiving supplementary pyridoxine

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/0k225f19x

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  • The bioavailability of vitamin B-6 from four selected foods was investigated in five men, aged 22 to 25 years, who were receiving a pyridoxine supplement. The subjects received a constant diet containing 1.34 mg of vitamin B-6 throughout this five-week study, except on Saturdays and Sundays when they ate their self-chosen diets. Starting on day 6 of week 1, following a five-day adjustment period, the subjects received orally 5-mg crystalline pyridoxine supplement daily, except on Tuesday and Thursday of each week. On these two days, the subjects were given orally 0 mg or 2 mg of crystalline pyridoxine, or test doses of bananas, filberts, soybeans and beef which contained around 2 mg of vitamin B-6. Vitamin B-6 was determined by microbiological assay with Saccharomyces uvarum. Vitamin B-6 bioavailability in the test food was determined by comparing 24-hour urinary total vitamin B-6 in response to the test food doses to that excreted following a 2-mg crystalline pyridoxine dose in each subject. Compared to the 100 percent bioavailability of the 2-mg crystalline PN dose, the average vitamin B-6 bioavailability in bananas was 115 + 32% and that in filberts, soybeans and beef was 93 + 8%, 73 + 20% and 87 + 7%, respectively. The metabolism of vitamin B-6 in pyridoxine-supplemented subjects was also investigated by measuring changes in plasma total vitamin B-6 which increased and was stablized after three weeks of pyridoxine supplementation. It was concluded that urinary total vitamin B-6 in pyridoxine-supplemented subjects can be used as a measure of vitamin B-6 bioavailability from test food doses.
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