Graduate Thesis Or Dissertation
 

Marker-assisted selection for resistance to potato virus Y

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/0k225f473

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  • Potato Virus Y (PVY) imposes serious limitations on potato yield, quality and tuber seed production. Traditional PVY screening requires artificial inoculation under controlled conditions followed by ELISA to test for PVY. This method is very tedious and time consuming thus prohibiting the screening of large segregating populations. The genes RYadg from S. tuberosum ssp. andigena and Rysto from S. stolonferum provide extreme resistance to PVY. The objective of this work is to assess the usefulness of molecular markers for determining the allelic configuration of parental material containing R.yadg and Rysto genes and as an early selection tool for predicting PVY resistance. To achieve this, two segregating populations for RYadg and Rysto were screened with molecular markers, inoculated artificially and tested for PVY by ELISA. Ninety-six percent of the segregating lines for the RYadg gene showed coincidence between results for molecular markers and ELISA at 40 days after inoculations. Both ELISA and molecular marker results fit a 1:1 (resistant:susceptible) segregation ratio indicating the presence of Ryadg as a simplex. In the population segregating for Rysto only 84% of the segregating lines showed coincidence between results for molecular markers and ELISA at 40 days after inoculations. The molecular markers results fit a 1:1 segregation ratio whereas the ELISA results indicated that a second gene/allele was likely providing resistance to PVY. Markers associated with RYadg and Rysto were successfully used for Marker-Assisted Selection in the Pacific NW Potato Breeding program. In addition, advanced potato clones were evaluated for presence Of Ryadg and Rysto PVY resistance sources. The lines LBR2, B071 8-3 and EGA97061-4 showed patterns of resistance for PVY associated with RYadg based on molecular marker evaluations. Additional sources of PVY resistance, not detected with the markers used in this study, are likely present in other advanced potato germplasm evaluated.
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