Graduate Thesis Or Dissertation

 

An electrophoretic analysis of the coelomic and blood sera of the developing and metamorphosing bullfrog tadpole (Rana catesbeiana) Public Deposited

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  • Albumin, as a percent of total proteins in both the coelomic serum and the blood serum, was determined for four groups of developing Rana catesbeiana tadpoles by cellulose acetate electrophoresis. Also, using Folin phenol reagent, the total protein of each the blood serum of metamorphosing tadpoles and the coelomic serum was determined, along with the coelomic fluid weights relative to the total body weights of these tadpoles. Coelomic fluid cells were identified and percentages for them calculated. Blood protein levels (1.68±SE 0.01 g/100 ml) are significantly higher than the coelomic fluid proteins (1.04±SE 0.01 g/100 ml) in metamorphosing animals. The percentage of albumin of total protein in the coelomic fluid (Group I, 18.3±2.3; II, 21.5±1.07; III, 22.7±0.68; IV, 31.3±1.25) is significantly higher than that in the blood (Group I, 18.3±2.30; II, 18.2±0.82; III, 20.1±0.82; IV, 27.7±1.16) for the last three Developmental Groups (Gosner stages 32-46). Percentages of albumin in the blood and in the coelomic fluid did not appear to increase at p<0.05, except in the last Group (IV). The coelomic fluid weight decreased during the last five metamorphic stages both absolutely, and also relative to tadpole weight. The majority of the coelomic cells identified were lymphocytes (51-85%). Monocytes accounted for 3-14%, and eosinophiles, erythrocytes, histiocytes, neutrophiles and thrombocytes combined, accounted for 4-23%.
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