Graduate Thesis Or Dissertation
 

An electrophoretic analysis of the coelomic and blood sera of the developing and metamorphosing bullfrog tadpole (Rana catesbeiana)

Public Deposited

Downloadable Content

Download PDF
Request Accessible Version
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/0k225f740

Descriptions

Attribute NameValues
Creator
Abstract
  • Albumin, as a percent of total proteins in both the coelomic serum and the blood serum, was determined for four groups of developing Rana catesbeiana tadpoles by cellulose acetate electrophoresis. Also, using Folin phenol reagent, the total protein of each the blood serum of metamorphosing tadpoles and the coelomic serum was determined, along with the coelomic fluid weights relative to the total body weights of these tadpoles. Coelomic fluid cells were identified and percentages for them calculated. Blood protein levels (1.68±SE 0.01 g/100 ml) are significantly higher than the coelomic fluid proteins (1.04±SE 0.01 g/100 ml) in metamorphosing animals. The percentage of albumin of total protein in the coelomic fluid (Group I, 18.3±2.3; II, 21.5±1.07; III, 22.7±0.68; IV, 31.3±1.25) is significantly higher than that in the blood (Group I, 18.3±2.30; II, 18.2±0.82; III, 20.1±0.82; IV, 27.7±1.16) for the last three Developmental Groups (Gosner stages 32-46). Percentages of albumin in the blood and in the coelomic fluid did not appear to increase at p<0.05, except in the last Group (IV). The coelomic fluid weight decreased during the last five metamorphic stages both absolutely, and also relative to tadpole weight. The majority of the coelomic cells identified were lymphocytes (51-85%). Monocytes accounted for 3-14%, and eosinophiles, erythrocytes, histiocytes, neutrophiles and thrombocytes combined, accounted for 4-23%.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 24-bit Color) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Accessibility Feature

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details ThornburgKentL1972.pdf 2017-08-23 Public Download