Physiology of the corpus luteum of the rat Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1831cp355

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  • Rat luteal cells from functional and non-functional corpora lutea (corpora lutea rendered non-functional by hypophysectomy) were preserved for light and electron microscopy in Bouin's and glutaraldehyde-osmium fixation respectively. Material for electron microscopy was dehydrated with ethanol, embedded in Araldite and stained with lead hydroxide or uranyl acetate-lead citrate. Examination of functional and three day non-functional luteal cells by the electron microscope revealed no detectable differences in ultrastructure. The appearance of dense bodies demarcated the one week non-functional luteal cells which also contained a more dilated endoplasmic reticulum and more spherical mitochondria. Luteal cells, non-functional for two weeks, were similar to one week non-functional cells except for more exaggerated structural differences. Dilation of the endoplasmic reticulum may have been due to poor fixation. Luteal cells which were non-functional for one month showed marked changes from functional luteal cells: i.e. mitochondria of low density and a sparse endoplasmic reticulum; dense bodies, and ribosomes were still evident. From these results, functional luteal cells cannot be distinguished from early non-functional cells by electron microscopy. Distinctions can be noted only after a period of three to seven days following cessation of function. Several hypotheses are presented in relation to the mechanism of action of luteotropic hormone.
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