Development of enteric protected vaccines for aquaculture Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1c18dh85g

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • At present all vaccines for fish are primarily delivered either by injection or immersion which introduces added stress and labor. A more attractive method of vaccine delivery is oral administration using an enteric protection system, Enteric Coated Antigen Microspheres (ECAMs), which can be utilized for a variety of antigenic forms. Relative efficacy of ECAMs was compared by inducing an antibody response in fish via injection (ip), immersion (im) or by ECAMs. Results showed that ECAMs were as effective as ip and im in inducing an antibody response to lipopolysaccharde and protein antigens. ECAMs were able to protect the protein antigen from gastric degradation. The ECAMs were employed to deliver a prototype vaccine for Renibacterium salmoninarum (Rs), the etiological agent of bacterial kidney disease. Upon characterization of the Rs antigens, one predominant cellassociated and extracellular protein (ECP), p57 was identified. The p57 molecule is elaborated in high concentrations in infected fish and exhibits pathogenic activitiesin vitro which appear to suppress the immune response. Our studies have revealed that a 370C incubation of R. salmoninarum cells decreased the amount of p57 by the induction of an autoproteolytic activity. This activity was exploited to produce a prototype vaccine that was delivered by intraperitoneal injection (ip) and demonstrated a significant increase in mean time of death upon challenge by injection. A second experiment was conducted using a natural exposure (bath challenge) and the heat treated, p57 -Rs cells were delivered using ECAMs and ip administration. The results proved that the route of immunization was critical with respect to natural exposure, as animals that received ECAMs with heat-treated p57 minus As cells demonstrated statistically significant reduction in the amount of ECP detected versus control. The decrease in ECP concentration is indicative of reduced Rs infection. Those animals vaccinated by ip showed no difference. Finally, in order to assess immunological memory induced by antigenic stimulation, an assay was developed that measured the production of cytokines induced by in vivo priming and subsequent in vitro exposure with the specific antigen.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Language
Digitization Specifications
  • Master files scanned at 600 ppi (256 Grayscale) using Capture Perfect 3.0 on a Canon DR-9080C in TIF format. PDF derivative scanned at 300 ppi (256 B&W, 256 Grayscale), using Capture Perfect 3.0, on a Canon DR-9080C. CVista PdfCompressor 3.1 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Linda Kathman(linda.kathman@oregonstate.edu) on 2008-05-14T20:51:06Z (GMT) No. of bitstreams: 1 Piganelli_Jon_D_1994.pdf: 1660586 bytes, checksum: 151795a96fbe7df757758161bb6c655b (MD5)
  • description.provenance : Approved for entry into archive by Linda Kathman(linda.kathman@oregonstate.edu) on 2008-05-14T20:49:09Z (GMT) No. of bitstreams: 1 Piganelli_Jon_D_1994.pdf: 1660586 bytes, checksum: 151795a96fbe7df757758161bb6c655b (MD5)
  • description.provenance : Made available in DSpace on 2008-05-14T20:51:07Z (GMT). No. of bitstreams: 1 Piganelli_Jon_D_1994.pdf: 1660586 bytes, checksum: 151795a96fbe7df757758161bb6c655b (MD5)
  • description.provenance : Submitted by Anna Opoien (aoscanner@gmail.com) on 2008-05-14T19:34:05Z No. of bitstreams: 1 Piganelli_Jon_D_1994.pdf: 1660586 bytes, checksum: 151795a96fbe7df757758161bb6c655b (MD5)

Relationships

In Administrative Set:
Last modified: 08/01/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items