Iron acquisition and its regulation in Nitosomonas europaea Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1c18dh90b

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  • Iron is absolutely necessary for the maintenance, the defense, the differentiation and last, but by no means least, the growth and cellular division of almost all living organisms including Nitrosomonas europaea, an ammonia oxidizing bacterium. Given the necessity of iron, it is not surprising that N. europaea evolved several irondependent proteins to perform many essential functions. Here, we used computational and molecular techniques to study the iron transport systems and their regulation in N. europaea. Despite its high iron requirement, N. europaea does not produce siderophores. However it can grow in media containing relatively low iron by lowering its cellular iron and heme contents and by efficiently allocating the available iron to required irondependent proteins. Similar to other Gram-negative bacteria, N. europaea is responding to iron limitation by increasing transcript levels of siderophore-dependent iron acquisition systems. In addition, transcripts of several hypothetical proteins with putative novel roles in iron acquisition were also present in higher levels when N. europaea is grown in iron limited conditions. Most of the genes involved in iron uptake that were mentioned above appear to be regulated by either iron alone or iron and Ferric Uptake Regulator (Fur) protein in N. europaea. In addition, it appears that N. europaea Fur also plays a role in maintaining a balance between acquiring enough iron and allocating it to various iron-dependent proteins. N. europaea is capable of taking up Fe³⁺-bound ferrichrome, ferrioxamine, pyoverdine via nitABC Fe-transporter. N. europaea might be having alternative means for transport of Fe³⁺ or Fe²⁺ ions across the cytoplasmic membrane and also Fe³⁺-bound to enterobactin and aerobactin siderophores.
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