Pineal and retinal acetylserotonin methyl-transferase activity in trout Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1c18dj57h

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  • Pineal acetylserotonin methyltransferase (ASMT) activities in rainbow trout were reported on by Hafeez and Quay (1971). The validity of certain assay parameters used by these researchers were investigated. With the exception of incubation temperature, all parameters studied proved to be quite valid. Temperature studies indicated a low stability for trout ASMT at 40°C. At this temperature enzymatic activity ceased after 20 minutes of incubation. Using the assay parameters developed from the previous experiments, levels of retinal ASMT were monitored in mature kamloops rainbow trout. Retinal ASMT activities of pinealectomized and sham-pinealectomized fish displayed no differences from the retinal ASMT activity of untreated controls. The same assay system was again utilized to investigate the possible diurnal ASMT activity changes in the pineal of steelhead trout smolts. A diurnal fluctuation in ASMT levels was discernible when total pineal protein was used to standardize the data. Although a similar trend could be seen when ASMT activity was expressed as a function of mean body weight, the pattern was not as consistent.
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  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2014-01-09T15:13:54Z (GMT) No. of bitstreams: 1 SmithJohnRobert1974.pdf: 636378 bytes, checksum: e39eb175be6582b8d69d2d53266855f0 (MD5)
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