Ultrastructural study of the embryo during germination involving douglas fir (Pseudotsuga menseizii Franco) seeds Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1g05fg38r

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  • The seeds of Douglas fir, a representative of the gymnosperm flora, were studied at six stages of germination to discern the structures of food reserves and to study ultrastructural changes occurring during germination in the embryo and resulting seedlings. The materials were fixed in three fixatives, dehydrated with ethanol series, embedded in Epon, and sectioned to 500-900 A in thickness, stained with uranyl acetate and lead citrate, and examined with a RCA EMU-2D electron microscope. Embryo cells contained many fat and protein bodies in addition to the nucleus, a few proplastids and mitochondria. Protein bodies first degraded to form a meshwork and then became completely solublized to form vacuoles in the cytoplasm. Fat bodies decreased in size and number at a rate slower than protein bodies and were utilized completely in root tissue. Amyloplasts increased at the early stages of germination when the fat and protein bodies started to degrade. At the later stages, the amyloplasts were difficult to observe. Other cellular organelles developed very rapidly with the advancement of germination; their ontagenetic sequences were comparable with plant materials of angiosperm.
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