|Abstract or Summary
- Previous research conducted by the Pharmacognosy department
at Oregon State University on Datura tatula indicated that
AMO 1618 and maleic hydrazide (MII) affected the growth and alkaloid
biogenesis of the plant. AMO 1618 treatment increased the
fresh and dry weights of the plants, but decreased the alkaloid
content. MH treatment resulted in an appreciable increase in plant
weight, an increase in total alkaloid content, but a decrease in
In view of these interesting effects onplant growth and alkaloid
formation, it was decided to continue the study into the second generation.
This investigation was conducted on seeds obtained from the various
groups of the aforementionedplants, i.e., those plants treated previously
with AMO 1618, those previously treated with MH, and
untreated (control) plants. Since the literature indicated that there
is an interaction between some growth retardants and gibberellic
acid (GA), part of the seeds from each group was soaked in a 50
ppm solution of GA and another portion soaked in distilled water.
This procedure provided six series each consisting of ten plants.
The six series of plants were randomized in the greenhouse, growth
observations were made and the plants were harvested when they
were about 67-days-old.
The growth rate, based on height measurements, was not
appreciably affected. However, fresh and dry weight data indicated
the following trends: the AMO 1618 second generation plants indicated
a marked reduction in fresh weights; the GA-seed treatment induced
an increase in the fresh weights of this group; a significant decrease
was noted in the dry weight of the leaves of this AMO 1618 group;
the GA-seed treatment induced a significant increase in the dry
weight of the capsules of this group; the GA-seed treatment induced
a decrease in the fresh weight of the control group; the GA-seed
treatment did not affect the fresh or dry weights of the MH group.
The alkaloid concentration of the AMO 1618 group, as well as
those in this group receiving a GA-seed treatment, was not affected.
However, considerable residual inhibition was noted in the concentration
of alkaloids in the MH group. This inhibition was markedly
reversed by GA-seed treatment. The alkaloid content per plant was
decreased in the second generation by both inhibitors. The GA-seed treatment of the control group reduced the total alkaloid content per
plant, whereas, GA-seed treatment caused a reversal of the residual
inhibition which was induced by each of the growth retardants.
The chlorophyll concentration was not affected by the various
treatments. The following pertinent data was obtained from the selective
solvent extraction of the leaves: the petroleum ether, alcohol
and water extracts were decreased by the residual effect of both
inhibitors; this decrease was reversed by GA seed treatment; two- to three-fold increases were noted in the ether soluble fractions of
plants treated with the inhibitors; GA-seed treatment induced a decrease
in the alcohol extract of the control group.