Graduate Thesis Or Dissertation
 

The effect of AMO-1618, maleic hydrazide and gibberellin-seed treatment on the second generation of Datura tatula Linn

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  • Previous research conducted by the Pharmacognosy department at Oregon State University on Datura tatula indicated that AMO 1618 and maleic hydrazide (MII) affected the growth and alkaloid biogenesis of the plant. AMO 1618 treatment increased the fresh and dry weights of the plants, but decreased the alkaloid content. MH treatment resulted in an appreciable increase in plant weight, an increase in total alkaloid content, but a decrease in height. In view of these interesting effects onplant growth and alkaloid formation, it was decided to continue the study into the second generation. This investigation was conducted on seeds obtained from the various groups of the aforementionedplants, i.e., those plants treated previously with AMO 1618, those previously treated with MH, and of untreated (control) plants. Since the literature indicated that there is an interaction between some growth retardants and gibberellic acid (GA), part of the seeds from each group was soaked in a 50 ppm solution of GA and another portion soaked in distilled water. This procedure provided six series each consisting of ten plants. The six series of plants were randomized in the greenhouse, growth observations were made and the plants were harvested when they were about 67-days-old. The growth rate, based on height measurements, was not appreciably affected. However, fresh and dry weight data indicated the following trends: the AMO 1618 second generation plants indicated a marked reduction in fresh weights; the GA-seed treatment induced an increase in the fresh weights of this group; a significant decrease was noted in the dry weight of the leaves of this AMO 1618 group; the GA-seed treatment induced a significant increase in the dry weight of the capsules of this group; the GA-seed treatment induced a decrease in the fresh weight of the control group; the GA-seed treatment did not affect the fresh or dry weights of the MH group. The alkaloid concentration of the AMO 1618 group, as well as those in this group receiving a GA-seed treatment, was not affected. However, considerable residual inhibition was noted in the concentration of alkaloids in the MH group. This inhibition was markedly reversed by GA-seed treatment. The alkaloid content per plant was decreased in the second generation by both inhibitors. The GA-seed treatment of the control group reduced the total alkaloid content per plant, whereas, GA-seed treatment caused a reversal of the residual inhibition which was induced by each of the growth retardants. The chlorophyll concentration was not affected by the various treatments. The following pertinent data was obtained from the selective solvent extraction of the leaves: the petroleum ether, alcohol and water extracts were decreased by the residual effect of both inhibitors; this decrease was reversed by GA seed treatment; two- to three-fold increases were noted in the ether soluble fractions of plants treated with the inhibitors; GA-seed treatment induced a decrease in the alcohol extract of the control group.
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file details MasoudAsaadN1963.pdf 2017-08-09 Pubblico Scaricare