Characterization of phenolics in Marion and Evergreen blackberries Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1n79h722c

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  • Frozen Marion (Rubus sp. hyb) and Evergreen blackberries (Rubus laciniatus) (whole berries, berries without seeds, and seeds alone) were acetone/ chloroform extracted. Phenolics were isolated using C₁₈ cartridges and polyamide resins, then characterized and measured by reversed-phase HPLC/diode array spectral techniques. The Polyamide isolated fractions of Marion berries contained four quercetin glycosides, one kaempferol glycoside, three acylated quercetin glycosides, (-)-epicatechin, and eight ellagic acid derivatives. Polyamide isolated fractions of Marion seeds had several procyanidins (including (+)-catechin and (-)-epicatechin), 11 tentative ellagitannins, and eight ellagic acid derivatives. (-)-Epicatechin and ellagic acid derivatives were the primary phenolics in blackberry fruits and seeds. Flavonols were the major component in whole berries, while procyanidins and ellagic acid derivatives were the primary phenolics in seeds. No flavonols were found in seeds. The phenolic profiles of Marion and Evergreen were qualitatively similar. Evergreen berries contained higher level of (-)-epicatechin, ellagic acid derivatives, and flavonols than Marion berries. Evergreen seeds were also higher in procyanidin content than Marion seeds, while Marion seeds were higher in ellagic acid derivatives. No ascorbic acid was found in any extracts. The total monomeric anthocyanin content of Marion berries (225 mg of Cyanidin 3-glucoside/100g) was 1.5 times higher than that of Evergreen berries (154 mg/100g). However, the total phenolics, total monomeric anthocyanins, oxygen radical absorbing capacity (ORAC), and ferric reducing antioxidant power (FRAP) values of Marion and Evergreen berries were not statistically different at the significance level 0.05. The total phenolics, ORAC, and FRAP values of Marion berries were 844 mg of gallic acid equivalent (GAE)/100g, 35.5 μmole of Trolox equivalent (TE)/g, and 79.1 μmole of TE/g, respectively. The total phenolics, ORAC and FRAP values of Evergreen berries were 822 mg of GAE/100g, 34.3 μmole of TE/g, and 74.2 (μmole of TE/g, respectively. Over all, Marion blackberries were higher than Evergreen blackberries with respect to phenolic content and antioxidant capacity. This study confirmed that blackberries contributed a significant source of phenolic antioxidants that may have potential health effects. Blackberry seeds, accounting for 5.0% of the weight of Marion berries and 5.6% of Evergreen berries, had very high phenolic content and antioxidant capacity; therefore they may be a potential source for nutraceuticals and natural antioxidants.
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