Growth characteristics and metabolism of Beggiatoa Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1r66j376q

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  • The addition of catalase to culture medium increased the period of viability of Beggiatoa from one week to two months. Addition of catalase also produced a marked increase in cell yield and enzyme activity. The addition of cysteine or hydrogen sulfide to the growth medium or the use of semi-solid medium stimulated growth. Oxygen was found to be required for growth, but carbon dioxide was not produced. Citrate and sulfite were inhibitory to growth whereas malate and acetate stimulated growth. Glucose and thiosulfate were not oxidized, and cytochromes were not detectable by spectrophotometric analysis. Cells grown on agar surfaces in the absence of catalase exhibited an absorption peak characteristic of peroxides. This absorption peak was removed by addition of catalase during or after growth. A proposed system which would permit acetate incorporation into four carbon compounds without the presence of key enzymes of the citric acid cycle or glyoxylate bypass was described. In this system acetyl-CoA is condensed with glyoxylate to form malate which, in turn, is converted to oxaloacetate. Oxaloacetate then reacts with glutamate to produce α-ketoglutarate, which is subsequently converted to isocitrate. Cleavage of isocitrate produces glyoxylate and succinate to complete the cycle. Citrate and fumarate are not involved in the proposed cycle. Fumarase, aconitase, catalase, citratase, pyruvate kinase, enolase, phosphoenopyruvate carboxylase, lactic dehydrogenase, α-ketoglutarate dehydrogenase and condensing enzyme were not detectable in crude extracts of Beggiatoa. Succinate was oxidized by a soluble enzyme not associated with an electron transport particle. Sulfite, sulfide and ascorbic acid oxidation appeared to be correlated with peroxide production by flavoproteins during oxidation of organic substrates.
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