Allozyme genetics and mating systems of Cuphea Laminuligera and Cuphea lutea Public Deposited

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  • Cuphea laminuligera Koehne and Cuphea lutea Rose in Koehne are undomesticated species having potential as oilseed crops. Genetic markers and mating systems have not been described for these species. Our objectives were to survey these species for allozyme variation, analyze the segregation and linkage of polymorphic loci, estimate autofertility and outcrossing rates, and investigate the effect of plant density on outcrossing rate. We analyzed allozyme variation among 11 F2 populations of C. laminuligera and one F2 population of C. lutea. Both species were assayed for aconitase, diaphorase, esterase, fluorescent esterase, glutamine oxaloacetate transaminase, malate dehydrogenase, menadione reductase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, and shikimate dehydrogenase activity. Cuphea laminuligera was also assayed for phosphoglucomutase activity. We observed 14 polymorphic loci and two monomorphic loci in C. laminuligera. Variation was observed within and between parental populations. Observed segregation ratios were generally not significantly different (P > 0.05) from expected ratios. We observed three polymorphic loci and at least 10 monomorphic loci in C. lutea. We found no within population variation in parental populations. Observed segregation ratios were not significantly different from expected ratios and linkage was not detected. Autofertility was estimated using plants isolated under insect-proof cages. The mean seed set per flower for C. laminuligera was 0.00 in 1986 and 1987. The mean seed set per flower of C. lutea was 4.75 and 4.64 in 1986 and 1987, respectively. Outcrossing rates (t) were estimated for four populations of C. laminuligera and three populations of C. lutea using allozyme phenotypes of open-pollinated individual plant families. Populations were grown at low (1.0 x 1.0 m) and high (0.04 x 0.3 m) density. Mating system parameters were estimated using the mixed mating model. Multilocus estimates of t ranged from 0.83 to 0.98. and 1.00 to 1.01 for low and high density populations of C. laminuligera, respectively. Multilocus estimates of t ranged from 0.17 to 0.26 and 0.36 to 0.54 for low and high density populations of C. lutea, respectively. C. laminuligera is strongly allogamous; however, we observed selfing rates as high as 17%. C. lutea is predominantly autogamous, but outcrossing rates occasionally exceeded 50%. Outcrossing rates were greatly affected by plant density.
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