An electrophoretic investigation of nucleoprotein organization in yeast chromatin Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1z40kw451

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • I have investigated nucleoprotein organization in chromatin of the yeast, Saccharomyces cerevisiae. My approach has been to prepare insoluble, minimally per turbed chromatin from a mitochondrial DNA-free strain of yeast, using a number of new techniques, and then digest this substrate with micrococcal nuclease. The soluble products are electrophoresed on a newly devised high resolution native nucleoprotein gel; DNA and protein constituents of the resolved complexes are analyzed by means of second dimension electrophoresis. I find that yeast nucleoproteins resolve into two bands for each DNA size class, lack a chromatosome, display a distinctive non-random pattern of nuclease excision for dinucleosomes and larger species, and have a markedly reduced complement of mononucieosomes when prepared in this manner. A single non-core protein of about 32,000 daltons appears to be bound to oligonucleosomes, but in modest amount, and primarily on the trinucleosome. A very prominent 40 kilodalton protein is selectively released by RNase, but is not associated with oligonucleosomes during native electrophoresis. These findings are discussed, with emphasis on nucleosome-nucleosome interactions and mononucleosome stability.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Submitted by Katy Davis (kdscannerosu@gmail.com) on 2013-07-25T15:50:41Z No. of bitstreams: 1 SzentGyorgyiChristopherS1983.pdf: 2758758 bytes, checksum: 0de481ed52b1eb73a3feea31b0c3b8c1 (MD5)
  • description.provenance : Made available in DSpace on 2013-07-30T23:22:23Z (GMT). No. of bitstreams: 1 SzentGyorgyiChristopherS1983.pdf: 2758758 bytes, checksum: 0de481ed52b1eb73a3feea31b0c3b8c1 (MD5) Previous issue date: 1982-10-15
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-07-29T16:23:50Z (GMT) No. of bitstreams: 1 SzentGyorgyiChristopherS1983.pdf: 2758758 bytes, checksum: 0de481ed52b1eb73a3feea31b0c3b8c1 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-07-30T23:22:23Z (GMT) No. of bitstreams: 1 SzentGyorgyiChristopherS1983.pdf: 2758758 bytes, checksum: 0de481ed52b1eb73a3feea31b0c3b8c1 (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items