Chitinase production of Arthrobacter sp. BN2 Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1z40kw469

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  • A chitinase producing bacterium was isolated from soil and identified as a species of Arthrobacter. The organism grew well at temperatures between 27 and 33.5 C, and the chitinase production was highest at 25.5 C. As the temperature increased above the optimum point, the level of enzyme production rapidly decreased, while the rate of decrease was slower at lower temperatures. Optimum pH for both growth and enzyme production was 6.4. Colloidal crab chitin or powdered crab chitin was an excellent substrate for chitinase production and the addition of Fe ⁺⁺ and Co ⁺⁺ enchanced the enzyme production. Under optimum conditions the organism produced 90 units (μg N-acetylglucosamine/min/ ml culture) of extracellular chitinase in 4 to 8 days. Chitinase of the Arthrobacter sp. is an inducible enzyme and at least 3% of chitin in the growth medium was necessary for maximum production of the enzyme. Chitinase production by the Arthrobacter sp. was greatly increased when the organism was grown with molds (5-fold with Aureobasidium pullulans, 12-fold with Penicillium sp., and 8-fold with Aspergillus niger and Phanerochaeta chrysosporum), but cultivation with other bacteria, actinomycetes, and yeasts had no effect.
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