Development of mucosal vaccines for avian influenza A virus composed of recombinant Lactococcus lactis and Streptococcus gordonii Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/2514np76t

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  • Novel mucosal vaccines (LL-M2e, LL-HAe, SG-HAe) were constructed from live, non-pathogenic Lactococcus lactis or Streptococcus gordonii that express conserved regions of HA or M2 antigens from avian influenza virus (AIV) A. All three vaccines evoked antigen-specific serum immunoglobulin G (IgG) responses in vaccinated chickens. The addition of the adjuvant cholera toxin B (CTB) increased the antigen specific IgG response. Fecal IgA and cell mediated immune responses were not detectable in the chickens. LL-M2e provided significant protection against lethal challenge compared to mock-vaccinated chickens. None of the chickens vaccinated with the control strain (LL-PIP) or mock-vaccine survived lethal challenge past 5 days, whereas 2 of the 12 chickens vaccinated with LL-M2e survived at least 7 days post-infection. An M2e-specific serum IgG concentration of 1100 ng/ml was found to be the minimum required for increased protection against AIV infection. SG-HAe, but not LL-HAe, provided a significant increase in the number of chickens that survived lethal challenge compared to control-vaccinated birds. However, no correlation between HAe-specific serum IgG and survival was found.
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