Surfactant interactions with recombinant factor VIII at the air/water interface Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/2514nq08w

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  • The surface tension kin'ti4tnd ste'dy state behavior exhibited by mixtures of selected surfactants with a recombinant Factor VIII (rFVIII) were evaluated with automated interfacial tensiometry. In particular, fresh solutions of rFVIII with different concentrations of Tween 80, sodium dodecylsulfate (SDS), or dodecyltrimethylammonium bromide (DTAB) were prepared and their surface tension decrease monitored continuously by the DuNuoy ring and Wilhelmy Plate methods. The range of surfactant concentrations studied included several orders of magnitude, with values below and above the critical micelle concentration (CMC). The steady state behavior of rFVIII-Tween mixtures was adequately described by current theory for mixtures of random chain protein molecules with small surfactants at equilibrium. However this theory did not clearly explain the behavior of rFVIII-SDS and rFVIII-DTAB mixtures. Rather, the strong protein-surfactant association resulting in higher surface activities than that observed for pure surfactant or protein alone at each concentration, with full displacement of protein from the interface at high surfactant concentration. Analysis of protein-surfactant surface tension kinetics using a simple firstorder rate equation produced two rate constants (K₁ and K₂) in each case, presumably reflecting initial adsorption and subsequent rearrangement (including protein-surfactant dissociation) at the interface. The values of these rate constants differed for each rFVIIIsurfactant mixture, in a manner consistent with surface tension data.
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