| Abstract |
- The primary purpose of this investigation was to characterize
the lipids of the spores and vegetative cells of Cl. botulinum. A
second purpose was to explore the possibility that lipids might serve
as a means of differentiating the chiefly proteolytic Cl. botulinum
type B from the nonproteolytic Cl. botulinum types E and F.
The total lipid extracted accounted for 3.7%, 3.3%, 2.0%,
2.7%, and 3.0% of the dry weight of Cl. botulinum vegetative cell
types 61E and F; and spore types 61E, F, and 115B, respectively.
The fatty acids were analyzed in the form of their methyl esters
by gas-liquid chromatography. Infrared spectroscopy, mercuric
acetate fractionation, and silver nitrate-thin layer chromatography
served as complementary means of analysis. The total
fatty acids included straight chain saturated, unsaturated, and cyclopropane
acids. Palmitic and myristic were the predominant acids
in both the spores and vegetative cells of types 61E, F, and 115B.
Together, they made up over 50% of the total fatty acids.
Unsaturated acids were the second major group. These were
primarily 7, 8-tetradecenoic, 9, 10-hexadecenoic, 7, 8-hexadecenoic,
11, 12-octadecenoic, and 9, 10-octadecenoic acids, Types E and F
possessed an 18-carbon diunsaturate, which was not found in the
vegetative cells or spores of type 115B. However, insufficient
quantities prevented its further characterization. The vegetative
cells and spores also contained C₁₅, C₁₇, C₁₉ cyclopropane fatty
acids as adjudged by their infrared spectra and gas-liquid chromatographic
behavior.
The phospholipids accounted for approximately 60% of the total
lipids in the vegetative cells and 40% of that in the spores. The primary
phospholipid was phosphatidylethanolamine. Qualitative tests
for plasmalogens, glycolipids, and phosphatidic acid were positive
for both spores and vegetative cells.
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| Additional Information |
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Previous issue date: 1967-10-27
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