Techniques and media for excised tip culture of Vinifera cultivars Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/2b88qf531

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  • Excised apical stem tips (2-5 mm) of grape cultivars grew well on Murashige and Skoog's (1962) medium for tobacco callus. Additions of glycine were not essential but gave a slight stimulus to growth. Naphthalene acetic acid slightly reduced growth rates but reductions were not statistically significant. Kinetin and coconut milk were both significantly detrimental to the growth of the excised tips. Tips that developed callus following treatment with IBA (indolebutyric acid) made more growth (significant at .01 probability) than tips without a callus mass at the cut end. Indolebutyric acid was necessary to root production in the second sub-culture on agar medium. Elongated stem tips with roots, transferred to a liquid medium on a filter paper bridge, grew vigorously and produced abundant roots. These young plants were later transferred to greenhouse soils and developed to mature, healthy plants typical of the cultivar.
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