The relationship between plasminogen activator production and various aspects of bovine embryo development in vitro Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/2f75rb627

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  • The relationship between plasminogen activator (PA) production and cell stage, cell number and changes in overall diameter and zona pellucida thickness for bovine embryos developing in vitro was determined. Late morulae to blastocysts (n=80) were collected nonsurgically from naturally mated, estrous-synchronized and superovulated crossbred beef cows. Embryos were cultured, one embryo per 25-μl microdrop, for 6 d. At 24-h intervals, embryos were evaluated for cell stage and transferred to fresh microdrops, media were recovered for PA analysis, and embryo diameters and zona pellucida thicknesses were measured with an ocular micrometer. Plasminogen activator production was determined using a caseinolytic assay with urokinase as the standard. Changes in diameter, zona pellucida thickness and PA production per 24-h interval for each embryo were plotted and the graphs cut out and weighed. Sixty-one (76%) embryos completed the hatching process. Total PA production was positively correlated to embryonic size (r=.40; P<.001), developmental stage (r=.35; P=.001) and cell number (r=.35; P=.005) and negatively, but weakly, correlated to zona pellucida thickness (r=-.13; P=.267). Hatched embryos produced more total PA than embryos that did not hatch (.140 ± .011 vs .070 ± .019 g; P<.01). The results suggest, as embryonic size and cell number increases and cell stage progresses, bovine embryos liberate more PA.
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