Glucose catabolism in normal and fluoride injured mung bean (Phaseolus aureus) seedlings Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/2j62s866s

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  • Studies in other laboratories have established the presence of the Embden-Meyerhof-Parnas (EMP) pathway and the pentose phosphate (PP) pathway in plant tissue. Research in this laboratory with mung bean (Phaseolus aureus) seedlings has confirmed the presence of these two pathways as well as the glucuronic acid (GA) pathway. However, no reliable method for estimating the relative participation of these concurrent pathways in plants has existed. In this study the primary pathways of glucose catabolism in ten-day old mung bean seedlings were identified. A new approach, designated as the catabolic rate method, was developed to estimate the relative participation of three concurrent catabolic pathways of glucose. Then the effect of fluoride on glucose catabolism was examined using the catabolic behavior data. A continuous substrate feeding technique was developed in connection with the catabolic rate method thereby permitting the collection of radiorespirometric data on mung bean seedlings respiring at a metabolic steady state. These data in turn provided reliable information on the rate of evolution of respiratory ¹⁴CO₂evolved from intact mung bean seedlings catabolizing ¹⁴C specifically labeled glucose substrates in the dark. Comparative examination of the rate data on ¹⁴CO₂ formation made it possible to calculate the extent of participation of individual catabolic pathways of glucose. It was found that in ten-day old mung bean seedlings glucose was catabolized via the EMP pathway, the PP pathway, and the GA pathway to the extent of 74%, 17%, and 9% respectively. Fluoride ion at a concentration of 20 ppm in the intact plant was found to inflict a severe inhibitory effect on the EMP-pyruvate decarboxylation pathway. It was of even greater interest to observe that in the presence of fluoride the PP pathway and the GA pathway were playing more important roles in over-all glucose catabolism of intact mung bean seedlings.
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