Graduate Thesis Or Dissertation

Advances in Freshwater Cyanobacterial Genomics

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  • Limnology is undergoing a transition to high-throughput -omic analysis of freshwater bacterial communities. An important first step in making the transition is to characterize several genomes that can be used as references to guide metagenome assembly and analysis. Here I characterize four new freshwater cyanobacterial genomes, a pair of lake community metagenomes, and a temperate phage. The Anabaena sp. WA102 genome is sequenced with long-read sequencing to finished status, unique structural features of the genome are analyzed, a comparative genomic analysis with other members of the Nostocaceae is carried out, and its capacity to produce anatoxin-a (and related toxin variants) is assessed. Two metagenomes of the cyanobacterial bloom community in Anderson Lake, Jefferson County, Washington State, USA are analyzed, using the finished Anabaena sp. WA102 genome to identify the dominant anatoxin-a-producing strain in the metagenomes and determine that the dominant cyanobacterial strain is nearly identical and likely clonal between blooms in 2012 and 2013 Two new Nostocaceae genomes, Anabaena sp. AL93 and Aphanizomenon sp. WA102, are sequenced and compared with Anabaena sp. WA102. A new freshwater Cyanobium species is isolated and its genome is sequenced, a temperate cyanobacterial phage that infects the strain is also isolated and sequenced and its integration into the host genome is characterized
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  • 2017-08-07 to 2018-03-24



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