Graduate Thesis Or Dissertation

Molecular taxonomy of Aeromonas

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  • DNA reassociations, measured by optical and double label membrane filter techniques, were used to assess genetic relationships among Aeromonas and enterobacteria. Aeromonads from human sources, food, fresh water, fish, and pet turtles were examined. The base compositions of three A. salmonicida isolates were 58% G+C while the base compositions of 19 other aeromonads ranged from 58-61% G+C. With A. hydrophila ATCC 7966 as reference, A. salmonicida exhibited 79-85% binding under non-stringent conditions (Tm-25°C). The A. salmonicida isolates were more related to ATCC 7966 than one-half of the A. hydrophila isolates. With _A. hydrophila ATCC 7966 as reference, the range in percent relative binding of aeromonads was 46-100% (Tm-25°C). Many cultures exhibited less than 70% binding with ATCC 7966 (Tm-25°C). DNA-DNA homologies among ten environmental and human isolates of Aeromonas show that there are no correlations between percent relative binding to the A. hydrophila reference and environmental source or human pathogenicity. The genome sizes of ten aeromonads were compared and found to vary by approximately 30%. Percent relative bindings ranging from 20-40% were exhibited between Aeromonas reference strains and Citrobacter, Enterobacter, Klebsiella, Shigella, Arizona, and Salmonella. These studies detected the close ancestry between aeromonads and enterics and revealed the molecular heterogeneity within this group of gram-negative, fermentative, oxidase positive bacteria.
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