The effect of pH and ionic strength on the adsorption of β-lactoglobulin onto well-characterized silicon Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/37720h71n

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  • The effect of pH and ionic strength on the equilibrium adsorptive behavior of β-lactoglobulin onto hydrophobic and hydrophilic silicon surfaces was studied using ellipsometry. Plots of amount adsorbed (μg protein/cm²) as a function of protein concentration (mg/ml) exhibited attainment of plateau values beyond a protein concentration of 0.250 mg/ml. At a given pH and ionic strength, plateau values associated with hydrophobic surfaces were observed to be greater than those associated with hydrophilic surfaces. The Langmuir adsorption isotherm was chosen as the most appropriate model to represent the data and was used to compare results obtained under different experimental conditions. Effects of pH and ionic strength on protein adsorption at hydrophilic surfaces indicate that electrostatics played a major role, while pH and ionic strength effects on adsorption to hydrophobic surfaces reflect a greater importance of nonelectrostatic interactions.
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