Graduate Thesis Or Dissertation
 

The effect of pH and ionic strength on the adsorption of β-lactoglobulin onto well-characterized silicon

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/37720h71n

Descriptions

Attribute NameValues
Creator
Abstract
  • The effect of pH and ionic strength on the equilibrium adsorptive behavior of β-lactoglobulin onto hydrophobic and hydrophilic silicon surfaces was studied using ellipsometry. Plots of amount adsorbed (μg protein/cm²) as a function of protein concentration (mg/ml) exhibited attainment of plateau values beyond a protein concentration of 0.250 mg/ml. At a given pH and ionic strength, plateau values associated with hydrophobic surfaces were observed to be greater than those associated with hydrophilic surfaces. The Langmuir adsorption isotherm was chosen as the most appropriate model to represent the data and was used to compare results obtained under different experimental conditions. Effects of pH and ionic strength on protein adsorption at hydrophilic surfaces indicate that electrostatics played a major role, while pH and ionic strength effects on adsorption to hydrophobic surfaces reflect a greater importance of nonelectrostatic interactions.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details LueyJaKael.pdf 2017-10-19 Public Download