|Abstract or Summary
- The distributions of alkaline phosphatase, acid phosphatase,
non-specific esterases, and glycogen were described for the antennal
glands of the freshwater crayfish, Pacifastacus, and the marine crabs
Cancer and Pugettia.
Alkaline phosphatase is confined primarily to the luminal edge
of the labyrinth in all of the above forms whereas acid phosphatase
occurs in the coelomosac of Pacifastacus and Pugettia, and in the
labyrinth and bladder of all three forms. In Pacifastacus the protein,
horseradish peroxidase, injected into the hemocoel was subsequently
localized in the phagocytic, acid phosphatase containing cells of the
Both acid phosphatase and alkaline phosphatase are localized
at the luminal border of the labyrinth of the crayfish. This activity
may represent a single enzyme which is still active at both an
alkaline and acid pH.
A sexual dimorphism was observed in the labyrinth of Pacifastacus.
A very intense reaction for non-specific esterases sensitive
to 10⁻⁵M E 600 and insensitive to 10⁻⁵M eserine typified the
reaction in the male. The comparable reaction in the female was
much weaker and more diffuse.
The technique of gel electrophoresis was used to characterize
the dimorphism in the crayfish as well as those esterases in the
marine forms. The sexual dimorphism was observed in the antennal
glands of juvenile crayfish but was not present in the heart or hemolymph.
Eserine and E 600 sensitive esterases were observed in all
remaining areas of the antennal glands of Pacifastacus, the coelomosac,
labyrinth, and bladder of Pugettia, and labyrinth and bladder of
Glycogen is localized primarily in the coelomosac and bladder
of Pacifastacus and in the bladders of the marine forms.
It was concluded that the labyrinth of the crayfish may be the
site of a sex related function, involving the metabolism and excretion
of a metabolite or hormone inherent in the physiology of the male.
It seems feasible that glycogen stored in the proximal portions of
the antennal gland constitutes an energy source for these and other
functions since glycogen can be released into the lumen of the gland and reclaimed later in a more distal area.
No definite correlation could be established between the habitat
and histochemistry of the antennal gland of the decapods from divergent