The adsorption of human recombinant factor VIII in the presence of the nonionic triblock surfactant Pluronic® F-68 at the air-water interface Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/3j333544j

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • The adsorption behavior of a human recombinant Factor VIII (rFVIII) in the presence of the nonionic, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock surfactant Pluronic® F-68 was evaluated by dynamic air-water tensiometry. Surface tension kinetics were recorded for F-68 in the absence of protein at concentrations ranging from 0 to 10,000 ppm. The rate and extent of surface tension depression in these samples was compared to dynamic tensiometry measurements recorded for F-68 solutions in the presence of rFVIII. Surfactants are used in the biopharmaceutical industry to stabilize therapeutic proteins from aggregation and adsorption loss during manufacturing, and this comparison suggested a substantially different mechanism for rFVIII stabilization by F-68 than that consistent with the kinds of surfactants commonly used in downstream processing. In particular, comparative kinetic data were consistent with the notion that with sufficient F-68 in solution, rFVIII did not adsorb at the air-water interface even though the interface was only partially occupied by F-68. We hypothesized that F-68 stabilized rFVIII in these tests by strong association with the protein itself, rather than by its adsorption and subsequent establishment of a steric barrier at the air-water interface. To test this hypothesis, turbidimetric measurements of rFVIII aggregation behavior in solution and zeta potential measurements of rFVIII adsorption on (1 μm) colloidal silica particles, each in the presence of selected concentrations of F-68, were evaluated. Results recorded with these approaches were inconclusive, but not inconsistent with the original hypothesis.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Keyword
Subject
Rights Statement
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2010-01-18T17:50:34Z (GMT) No. of bitstreams: 1 AlkhatibAveenK2010.pdf: 969026 bytes, checksum: 4442b9149dae2545a413e20bec621df9 (MD5)
  • description.provenance : Approved for entry into archive by Laura Wilson(laura.wilson@oregonstate.edu) on 2010-01-21T23:18:15Z (GMT) No. of bitstreams: 1 AlkhatibAveenK2010.pdf: 969026 bytes, checksum: 4442b9149dae2545a413e20bec621df9 (MD5)
  • description.provenance : Submitted by Aveen Alkhatib (alkhatia@onid.orst.edu) on 2010-01-15T18:21:01Z No. of bitstreams: 1 AlkhatibAveenK2010.pdf: 969026 bytes, checksum: 4442b9149dae2545a413e20bec621df9 (MD5)
  • description.provenance : Made available in DSpace on 2010-01-21T23:18:15Z (GMT). No. of bitstreams: 1 AlkhatibAveenK2010.pdf: 969026 bytes, checksum: 4442b9149dae2545a413e20bec621df9 (MD5)

Relationships

In Administrative Set:
Last modified: 08/14/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items